One-mark transfer pipette with a central bulb, slender stems and a drawn delivery tip
Shown configuration · pipette alone / pipette filler, liquid and printed class marking not shown

Volumetric glassware

Glass Pipette

A one-mark transfer pipette in borosilicate 3.3, calibrated to deliver at 20 °C within ISO 648 Class A limits of ±0.020 mL at 10 mL and ±0.030 mL at 25 mL.

Class A ±0.03 mL at 25 mLCalibrated to deliverBulb form for one volume
Stock status
In stock
Lead time
2–3 weeks
FOB price / pc
$0.80 - $1.25
MOQ
5,000 pcs

Final availability, dimensions, materials, assembled components and commercial terms are confirmed against the selected drawing and approved sample.

One volume, done well

Beats a graduated pipette at its single mark

Never pipette by mouth

A bulb or filler is part of the specification

Tip residue stays

Drain-out types are not blown out

Glass Pipette profile

The bulb exists so that one volume can be delivered better than a scale ever could.

One-mark and graduated types, delivery mode & class

Five distinctions that matter more than the price difference between them.

A one-mark pipette concentrates its whole capacity in a bulb and leaves only a narrow stem at the mark, so the meniscus is set precisely and the delivered volume is reproducible. Graduated pipettes trade that precision for flexibility. Knowing which of the two a method needs, and whether it is drain-out or blow-out, prevents most pipetting error.

01

One-mark and graduated pipettes are different instruments

A one-mark transfer pipette under ISO 648 delivers a single volume to Class A limits of ±0.020 mL at 10 mL and ±0.030 mL at 25 mL. A graduated pipette under ISO 835 delivers any volume within its range and carries a wider tolerance for doing so. Using a graduated pipette to deliver its full nominal volume gives away accuracy for flexibility that the method did not ask for.

02

Drain-out and blow-out are not interchangeable

Most pipettes are calibrated to drain, with the small amount remaining in the tip excluded from the stated volume; blowing that residue out over-delivers. Blow-out types, marked with frosted bands at the top, include it and must be blown out. Reading which type is in your hand takes a second and is the single commonest source of systematic pipetting bias.

03

Delivery time is part of the calibration

The tip is drawn so that free delivery takes a defined period, and rushing it by tilting or forcing leaves more liquid on the wall than the calibration assumes. Standard practice is to hold the pipette vertical, let it run freely, touch the tip to the wall of the receiving vessel and wait the specified moment before withdrawing.

04

Fillers changed the risk profile of the job

Mouth pipetting is not acceptable practice in any modern laboratory, and every specification here assumes a bulb or a pipette filler. This has practical consequences for the glass: the stem has to fit the filler cone, and a pipette that is too short for a deep vessel becomes a hazard when someone reaches for it with an unsuitable adapter.

05

Wetting behavior beats nominal tolerance in dirty glass

A pipette delivers its calibrated volume only if the liquid drains as an even film. Grease, fingerprints or dried residue break that film into droplets and cost far more than the ±0.03 mL of the class limit. Cleaning to a wettable surface, and rinsing with the solution before the working delivery, does more for accuracy than upgrading from Class B to Class A.

06

The calibration belongs to one temperature

Volumetric glassware is calibrated at a stated reference temperature, and both the liquid and the glass change volume away from it. Delivering a cold solution from a pipette calibrated at 20 °C introduces an error larger than the tolerance class the pipette was bought for, which is why the temperature of the liquid is recorded rather than assumed.

Application guide

One mark, one volume, delivered the same way every time.

A bulb pipette carries a single calibrated volume rather than a scale, which is what makes it more accurate than a graduated tube: there is only one line to read and one way to use it. These are development directions confirmed against the method.

Glass Pipette used for aliquots taken for analysis in a volumetric transfer setting

Volumetric transfer

Aliquots taken for analysis

For transferring an exact volume into a flask before dilution, where the whole determination rests on that aliquot being right. The single graduation and the fixed bulb volume remove the reading judgment a graduated pipette demands, which is why methods specify this type for the critical step.

  • Confirm the calibrated volume and class
  • Fill to the mark at eye level
  • Follow the stated delivery time
Glass Pipette used for how the last drop is treated in a delivery technique setting

Delivery technique

How the last drop is treated

Pipettes are calibrated either to deliver or to contain, and the difference is what happens to the liquid remaining in the tip. Using a to-deliver pipette as though it should be blown out introduces a systematic error into every result it touches.

  • Confirm to-deliver or to-contain
  • Never blow out a to-deliver pipette
  • Train operators on the technique
Glass Pipette used for a bulb or pump, never the mouth in a training new staff setting

Training new staff

A bulb or pump, never the mouth

For the first week of anyone joining a bench, where mouth pipetting is ruled out and they are handed a filler instead. The stem has to seat and seal in the fillers that laboratory already owns, because a pipette that will not hold on the bulb is exactly what tempts someone to improvise.

  • Confirm the stem fits the fillers used
  • Check the seal against the bulb
  • Never permit mouth pipetting

Applications describe workflow directions. Accuracy, calibration class and laboratory outcomes are confirmed for the selected program.

Common questions

Glass Pipette questions

Pipetting error is systematic far more often than it is random. These are the usual causes.

Should I blow out the last drop?

Only if the pipette says so. Most are calibrated to drain, and the residue left in the tip is excluded from the stated volume, so blowing it out over-delivers. Blow-out types carry frosted bands at the top of the stem and must be blown out. Checking which is in your hand takes a second and removes the commonest systematic bias in manual pipetting.

Is Class A worth the money for routine work?

Less often than laboratories assume. Grease, fingerprints and dried residue break the draining film into droplets and cost far more than the difference between Class A and Class B. Cleaning glassware to a properly wettable surface, and pre-rinsing with the working solution, generally buys more accuracy per pound than upgrading the class of the pipette.

Why does our delivered volume drift over a session?

Usually because the glass is progressively less wettable as it picks up residue, or because delivery technique changes as the analyst tires and starts withdrawing the tip early. Both are procedural. Re-rinsing between samples and standardizing the drainage and touch-off routine will remove more drift than any change to the glassware itself.

Does the calibration hold at any temperature?

It belongs to one temperature, normally 20 °C. Working well above or below that shifts both the glass and the liquid, so a pipette used in a warm laboratory is not delivering its marked volume. Note the working temperature with the result.

Next step

Send us the Glass Pipette brief.

Tell us the product, fill volume, closure preference and destination market. We will come back with the matching drawings, available configurations and a sample plan before anything is quoted.

  • 01Send the briefProduct, volume, closure and market
  • 02Get the optionsMatching drawings and configurations
  • 03Approve the sampleConfirm the pack before production